Phosphodiesterase induction in Dictyostelium discoideum by inhibition of extracellular phosphodiesterase activity
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چکیده
منابع مشابه
Induction by folate and folate analogs of extracellular and membrane-bound phosphodiesterase from Dictyostelium discoideum.
Folate stimulation is known to enhance Dictyostelium discoideum differentiation. During early differentiation, D. discoideum cells possess two classes of folate receptors which can be distinguished by their difference in specificity (R. J. W. de Wit, FEBS Lett. 150, 445-448, 1982). We investigated the type of receptor by which folate affects cell differentiation. Two independently regulated dev...
متن کاملThe role of phosphodiesterase in aggregation of Dictyostelium discoideum.
The role of cAMP phosphodiesterase in the cAMP-mediated aggregation of the cellular slime mould Dictyostelium discoideum was investigated with a morphogenetic mutant defective in phosphodiesterase production. Mutant cells become capable of aggregating normally when incubated in the presence of exogenous phosphodiesterase isolated from Idictyostelium or rat brain. Direct contact between enzyme a...
متن کاملCharacterization of a cAMP-stimulated cAMP phosphodiesterase in Dictyostelium discoideum.
A cyclic nucleotide phosphodiesterase, PdeE, that harbors two cyclic nucleotide binding motifs and a binuclear Zn(2+)-binding domain was characterized in Dictyostelium. In other eukaryotes, the Dictyostelium domain shows greatest homology to the 73-kDa subunit of the pre-mRNA cleavage and polyadenylation specificity factor. The Dictyostelium PdeE gene is expressed at its highest levels during a...
متن کاملEffects of calcium antagonists on cyclic AMP phosphodiesterase induction in Dictyostelium discoideum.
Previous studies have suggested that cyclic GMP and/or Ca2+ might function as second messengers in the induction by exogenous cyclic AMP of the cyclic AMP phosphodiesterase (PD) in Dictyostelium discoideum. To assess further the role of Ca2+ in PD induction we examined the effect on this process of a number of putative Ca2+-channel blockers. At relatively low micromolar concentrations, TMB-8, n...
متن کاملEvidence for a messenger function of cyclic GMP during phosphodiesterase induction in Dictyostelium discoideum.
Chemotactic stimulation of vegetative or aggregative Dictyostelium discoideum cells induced a transient elevation of cyclic GMP levels. The addition of chemoattractants to postvegetative cells by pulsing induced phosphodiesterase activity. The following lines of evidence suggest a messenger function for cyclic GMP in the induction of phosphodiesterase: (i) Folic acid and cyclic AMP increased cy...
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ژورنال
عنوان ژورنال: Developmental Biology
سال: 1982
ISSN: 0012-1606
DOI: 10.1016/0012-1606(82)90087-2